Individual Compounds of <i>Rhodoila quadrifida</i> Rhizomes and Roots: Isolating and Establishing Structure

Introduction. Medicinal plant raw materials, containing a wide range of various biologically active compounds, are considerable interest as likely source new, pharmacologically substances. One the promising plants is Rhodiola quadrifida (Pall.) Fisch. & C.A. Mey, since materials contain phenolic compounds and their derivatives, which cause anti-inflammatory, antioxidant anticarcinogenic effects. In addition, this has popularity in folk medicine dietary supplement. Aim. Isolation individual from rhizomes roots subsequent determination structure by NMR mass spectrometry for phytochemical profiling. Materials methods. Rhizomes , purchased pharmacy St. Petersburg (place time procurement according to information on package – Altai (near Barnaul), March 2019. The ethanol extract fractions (ethanol, hexane, butanol, water) obtained during sequential liquid-liquid extraction were examined LC-20 Prominence instrument (Shimadzu, Japan) with an SPD-M20A diode array detector determine chromatographic profile. most ethyl acetate fraction was purified column chromatography open glass columns sorbents different selectivity preparative [Smartlina (Knauer, Germany)] spectrophotometric detector. determined (Bruker Avance III 400 MHz, Germany) confirmed low-resolution [Flexar FX-15 (PerkinElmer, USA)]. Results discussion. During study, 7 isolated namely 3,4-dihydroxybenzoic acid, 4-hydroxybenzoic caffeic gallate, catechin, epicatechin tyrosol. These first . They major can characterize profile well direction pharmacological activity. Conclusion. For time, majority proved spectroscopy spectrometry, serve foundation further study species targeted its activity vitro vivo considering